Senior Scientist Next Generation Sequencing
Apply Now

Senior Scientist Next Generation Sequencing

King of Prussia, PA

The Center for Breakthrough Medicines is on track to be the largest single-site cell and gene therapy contract development and manufacturing organization in the world. Our comprehensive service offering accelerates speed to market through supply chain disruption mitigation strategies, end-to-end program management expertise and unique platform technology with the ability to follow a molecule from idea to commercialization. CBM’s integrated approach provides high quality and consistent end-to-end manufacturing capability including: Process and Analytical Development; Plasmid DNA production; Viral Vector Production of Lentivirus; AAV and Adenovirus; Testing and Analytics; Autologous, Allogeneic and Gene-edited Cell Therapy Bioprocessing; and Cell banking. CBM’s horizontal and vertical integration provides one source throughout a product’s life cycle.

CBM’s mission is to accelerate the delivery and affordability of lifesaving and life-changing therapies from discovery to market by offering a complete solution for the development and commercialization of cell and gene therapies.

CBM is located just outside of Philadelphia in the Heart of Cellicon Valley on The Discovery Labs campus set on 300 acres with 2.4 million square feet of life sciences lab and manufacturing space, allowing clients to scale their development and manufacturing needs without ever having to change sites, while providing employees a true community campus experience focused exclusively on life sciences.

The Discovery Labs is a Global Platform Company inclusive of CBM, The Discovery Labs Properties, UniteIQ incubator labs, and The Colony, a unique investment and resource model enabling groundbreaking therapies to be brought to life.


The Center for Breakthrough Medicines is seeking a talented and passionate Senior/Associate Principal Scientist to join its Analytical Development, Next-Generation Sequencing (NGS) team. As a Senior/Associate Principal Scientist, you will provide expertise in NGS-based analyses of DNA and RNA, while contributing to the development, optimization, and validation of clinical-grade assays to measure complex, blood-based analytes (e.g., whole-genome sequencing of cfDNA). You will work collaboratively with multiple teams to develop and optimize the assays in a regulated, high-throughput environment as well as identify ways to rapidly iterate and improve on existing methods to evaluate technologies that can enhance our end-to-end test performance. The successful candidate must be able to set and understand priorities in a local and matrixed environment, must be a team player with a collaborative spirit, and capable of building and maintaining networks within and outside the organization. 


  • Responsible for NGS wet lab and dry lab assay development, validation and transfer utilizing Illumina, PacBio and Oxford Nanopore platforms.
  • Improve cost, efficiency, and performance of existing wet and dry lab workflows
  • Research scientific literature and apply current and emerging NGS methods for advanced characterization of gene and cell therapy biologicals and outcomes of gene editing experiments.
  • Communicate and collaborate with R&D, Process Development and Manufacturing teams to identify NGS applications within current and future processes.
  • Develop, optimize, and validate NGS library preparation, quality control and sequencing methodologies.
  • Provide wet lab support to deliver custom and routine NGS projects.
  • Adapt new and existing NGS methods for high-throughput automation and liquid handling.
  • Perform primary NGS data analysis and data manipulation on Windows and Unix platforms.


  • PhD in biological sciences with 1-3 years of experience or Master’s/Bachelor’s degree with 5-10 years of experience required.
  • Deep understanding of molecular biology principles and proficiency in molecular biology procedures is required.
  • Direct experience with molecular biology assay development is required.
  • Knowledge of NGS applications for characterization of viral vectors, molecular cloning, gene transfer and gene editing is highly desired.
  • Practical knowledge of Windows molecular biology software (SnapGene, Vector NTI, IGV, USCS Genome Browser etc). Knowledge of Unix command line is a plus.
  • Understanding of advanced NGS techniques such as different types of RNA sequencing, chromatin sequencing, targeted resequencing, custom primer optimization, single cell technologies is a plus.
  • Understanding of modern vectorology, AAV and lentivirus production is a plus.
  • Ability to develop, improve and meticulously follow test methods and standard operating procedures (SOPs) in GxP environment.
  • Highly collaborative mindset, strong interpersonal skills, excellent verbal and written communication skills.

Compensation: TBD

Apply Now